Preparation of a Highly Purified Glycyl-L-leucine Dipeptidase from Pig Intestinal Mucosa.

Development of a radiochemical assay for glycyl-leucine dipeptidase in human B- and T-lymphocytes.

Recent technical advances in the isolation of human lymphocytes have made it possible to obtain preparations enriched in Bor T-cells (Wybran, 1973; Pellegrino et al., 1976). This in turn facilitates studies on the comparative enzymology of Band T-lymphocytes, which may be relevant to some leukaemic states. Studies on lysosomal enzymes, including acid phosphatase, /3-glucuronidase (Meusers et al...

Substrate specificity of a highly active dipeptidase purified from monkey small intestine.

Dipeptidase activity in the small intestinal mucosa during pregnancy and lactation in the rat.

1. Rats were mated and at weekly intervals during pregnancy and lactation, and after weaning, the dipeptidase activity in the supernatant fraction from small intestinal mucosa extracts was determined for two dipeptides: glycyl-L-leucine dipeptidase (EC 3.4.3.2) AND L-alanyl-L-glutamic acid dipeptidase. 2. Dipeptidase activity is found mainly in the soluble (spernantant) fraction of the mucosa h...

Highly purified synaptosomal membranes from rat brain. Preparation and characterization.

Synaptosomal plasma membranes were isolated from whole rat brain by a method derived from several existing ones. The procedure involves the sequential centrifugation of a crude mitochondrial fraction through a discontinuous sucrose gradient and a continuous cesium chloride gradient. The material is then subjected to osmotic lysis and centrifuged through an additional discontinuous sucrose gradi...

The specificity of leucine aminopeptidase.

Since Linderstr@m-Lang’s demonstration that the hydrolysis of Lleucylglycine (LG) is due to a distinctileucyl peptidase (l), various studies have shown that this enzyme is widely distributed (24) and requires for its activity the presence of Mn++ or Mg++ ions (2, 5). The enzyme has been regarded as a typical aminopeptidase (5), since it does not hydrolyze acylated compounds Such as benzoyl-L-le...